In vitro interactions between immunodominant membrane protein of lime witches’ broom phytoplasma and leafhopper vector proteins
نویسندگان
چکیده
Lime witches’ broom phytoplasma (16SrII, LWB) causes major losses to lime cultivation in Iran. The role of its immunodominant membrane protein (IMP) in the specific interaction with vector proteins was addressed. The carboxy terminal moiety of LWB IMP, coding the extracellular part of the protein, was expressed (LWB f∆IMP) as a tool to study IMP interactions with insect proteins. In preliminary affinity chromatography experiments, leafhopper vector proteins with different sizes interacted with LWB
منابع مشابه
Generation and expression in plants of a single-chain variable fragment antibody against the immunodominant membrane protein of Candidatus phytoplasma aurantifolia.
Witches' broom of lime is a disease caused by Candidatus Phytoplasma aurantifolia, which represents the most significant global threat to the production of lime trees (Citrus aurantifolia). Conventional disease management strategies have shown little success, and new approaches based on genetic engineering need to be considered. The expression of recombinant antibodies and fragments thereof in ...
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